SNORA59B Knockout cell line(SK-MES-1)

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location： Home > Products > KO Cell Lines > SNORA59B Knockout cell line(SK-MES-1)

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SNORA59B Knockout cell line(SK-MES-1) Order

SNORA59B Knockout cell line(SK-MES-1)
Catalog#
YKO-H165
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SNORA59B Knockout cell line(SK-MES-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SNORA59B Knockout cell line（SK-MES-1）
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
SNORA59B
Gene ID
677882
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%MEM+10% FBS
Accession
CVCL_E1C9(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SK-MES-1		 STR Info (Cell bank)
Cell Line:SK-MES-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 8888
D16S539 13131313
VWA 14141414
TH01 69.369.3
AMEL XYXY
TPOX 8888
CSF1PO 12121212
D12S391 2222
FGA 2024
D2S1338 1919
D21S11 2930
D18S51 1717
D8S1179 1314
D3S1358 1616
D6S1043 1219
PENTAE 511
D19S433 1414
PENTAD 1213
D1S1656 17.318.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SNORA59B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SK-MES-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SNORA59B gene knockout SNORA59B Knockout cell line（SK-MES-1） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SNORA59B Knockout cell line(SK-MES-1)
SNORA59B Knockout cell line(SK-MES-1)
Catalog#
YKO-H165
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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